Period 2. (01 March 2015 - 31 August 2016)

Participation to conferences/scientific meeting

  • 16/11/2015 : PERV (Porcine Endogenous Retrovirus) integration and expression in pancreatic islets and other tissues in neonatal and adult pigs. Dr. Nizar Mourad, UCL. Oral presentation at IPITA-IXA-CTS Joint Congress - Melbourne, Australia.
  • 18/11/2015: Beta-cell-specific expression of glucagon-like peptide 1 (GLP-1) and activated muscarinic receptor (M3R) improves pig islet secretory function. Dr. Nizar Mourad, UCL. Poster presentation at IPITA-IXA-CTS Joint Congress - Melbourne, Australia.
  • 18/11/2015 : Islets (II): improving islet function. Prof. Pierre Gianello, UCL. Invited Speaker : State-of-the-Art Workshop: Progress in Tissue & Cell Xenotransplantation at IPITA-IXA-CTS Joint Congress – Melbourne, Australia.
  • 19/11/2015 : Screening for PERV and known pathogens: Donor and recipient monitoring. Prof. Linda Scobie, GCU. Invited speaker : Zoonosis and Regulation at IPITA-IXA-CTS. Joint Congress - Melbourne, Australia.
  • 17/11/2015 : Assessment of PERV transmission across an alginate barrier for the encapsulation of porcine islets. Claire Crossan, GCU. Poster presentation at IPITA-IXA-CTS Joint Congress - Melbourne, Australia.
  • 16/11/2015 : Non-identical PERV profile observed in cloned pigs. Claire Crossan, GCU. Oral presentation at IPITA-IXA-CTS Joint Congress - Melbourne, Australia . 25/04/2015 : Research Day at GCU. Prof. Linda Scobie. Oral presentation.
  • 27-29/03/2015 : Generation of genetically modified mice using talen technology. Anita Fehér, Dr. Andràs Dinnyés, Biotalentum. Poster presentation at Hungarian Molecular Life Sciences 2015 - Eger, Hungary.
  • 9/09/2015 : A. Személyre szabott őssejtek“ − hogyan lehet bőr vagy vérmintából őssejteket, majd ideg- szív- és más sejttípusokat, szöveteket létrehozni emberben és állatokban. Dr. Andràs Dinnyès. Oral presentation at “A Szent István Egyetem Állatorvos-tudományi Karának Baráti Köre”. Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary.
  • 22 /05/2015 : Production of transgenic and Knock out pigs for xenotransplantation research: current results and future perspectives. Prof. Galli Cesare. Oral presentation at the scientific event “Seoul forum for Xenotransplantation” - Seoul, Korea.
  • 03/10/2015 : Cloning and endonuclease-mediated targeting of the first swine safe harbour locus to enhance long term expression of transgenes. Dr. Perota Andrea. Oral presentation at IGM Join Meeting - Pavia, Italy.
  • 11/11/2015 : Efficient generation of Gal-ko cell lines for SCNT by selection with biotin conjugated IB4-lectin attached to streptavidin-coated magnetic beads.
    Prof. Galli Cesare. Poster presentation at IPITA-IXA-CTS Joint Congress - Melbourne, Australia.
  • 13/12/2015 : Prof. Galli Cesare. Sharing Advances on Large Animal Models – SALAAM. Conference WG/MC/Scientific Meeting of COST Action BM1308 - Poznan, Poland.

Publications

  • Spizzo T, Denner J, Gazda L, Martin M, Nathu D, Scobie L, Takeuchi Y. First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes--Chapter 2a: source pigs--preventing xenozoonoses. Xenotransplantation. 2016 Jan-Feb;23(1):25-31. doi:10.1111/xen.12223. Epub 2016 Mar 4.
  • Denner J, Tönjes RR, Takeuchi Y, Fishman J, Scobie L . First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes--Chapter 5: recipient monitoring and response plan for preventing disease transmission. Xenotransplantation. 2016 Jan-Feb;23(1):53-9. doi: 10.1111/xen.12227. Epub 2016 Feb 26.
  • Crossans C, Mourad N, Scobie L, Gianello P. Assessment of PERV transmission across an alginate barrier for the encapsulation of porcine islets, 2016, in preparation.
  • Mourad N., Perrota A., Xhema D., Galli C, Gianello P. Transgenic expression of glucagonlike peptide 1 (GLP-1) and activated muscarinic receptor (M3R) significantly improves pig islet secretory function. Xenotransplantation. 2016, accepted.
  • Cooper DK, Matsumoto S, Abalovich A, Itoh T, Mourad N, Gianello P, et al. Progress in Clinical Encapsulated Islet Xenotransplantation. Transplantation 2016, 100(11), 2301-2308. doi: 10.1097/TP.0000000000001371
  • Cooper DK, Bottino R, Gianello P, Graham M, Hawthorne WJ, Kirk AD, et al. First update of the International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes-Chapter 4: pre-clinical efficacy and complication data required to justify a clinical trial. Xenotransplantation 2016, 23(1) , 46-52. DOI: 10.1111/xen.12226
  • Paulis M, Castelli A, Susani L, Lizier M, Lagutina I, Focarelli ML, Recordati C, Uva P, Faggioli F, Neri T, Scanziani E, Galli C, Lucchini F, Villa A, Vezzoni P. Chromosome transplantation as a novel approach for correcting complex genomic disorders. Oncotarget. 2015 6(34) , 35218-30. doi: 10.18632/oncotarget.6143.
  • Butler JR, Martens GR, Estrada JL, Reyes LM, Ladowski JM, Galli C, Perota A, Cunningham CM, Tector M, Joseph Tector A. Silencing porcine genes significantly reduces human-anti-pig cytotoxicity profiles: an alternative to direct complement regulation. Transgenic Res 2016 [Epub ahead of print] DOI 10.1007/s11248-016-9958-0
  • Perota A, Lagutina I, Quadalti C, Lazzari G, Cozzi E, Galli C. The Applications of Genome Editing in Xenotransplantation. J Genet Genomics. 2016 43(5) , 297-305. DOI: 10.1016/j.jgg.2016.04.012
  • Reynard O, Jacquot F, Evanno G, Mai HL, Salama A, Martinet B, Duvaux O, Bach JM, Conchon S, Judor JP, Perota A, Lagutina I, Duchi R, Lazzari G, Le Berre L, Perreault H, Lheriteau E, Raoul H, Volchkov V, Galli C, Soulillou JP. Anti-EBOV GP IgGs Lacking α1-3-Galactose and Neu5Gc Prolong Survival and Decrease Blood Viral Load in EBOVInfected Guinea Pigs , PLoS One. 2016 11(6). doi: 10.1371/journal.pone.0156775.

Publications (abstract)

IPITA-IXA-CTS 2015

  • 216 Non-identical PERV profile observed in cloned pigs Crossan C, Cruikshank V, Perota A, Duchi R, Lagutina I, Lazzari G, Mourad N, Takeuchi Y, Galli C, Gianello P, Scobie L. Xenotransplantation (2015) 22: S8
  • 348 Immune phenotype and IgG characteristics of Neu5Gc and alpha-1-3-gal double knock-out pigs Salama A, Conchon S, Perota A, Martinet B, Judor JP, Evanno G, Le-Bas Bernardet S, LeBerre L, Hervouet J, Minault D, Concordet JP, Dugast E, Vanhove B, Abadie J, Gaide N, Lagutina3 I, Duchi R, Lazzari G, Floch R, Duvaux O, Toutain PL, Maillasson M, Sachs DH, Gauthier O, Brouard D, Cozzi E, Blancho G, Perreault H, Bach JM, Galli C, Soulillou JP. Xenotransplantation (2015) 22: S38
  • 910 Beta-cell-specific expression of glucagon-like peptide 1 (GLP-1) and activated muscarinic receptor (M3R) improves pig islet secretory function Mourad N, Xhema D, Perota A, Galli C, Gianello P. Xenotransplantation (2015) 22: S176
  • 921 Efficient generation of Gal-ko cell lines for SCNT by selection with biotinconjugated IB4-lectin attached to streptavidin-coated magnetic beads Lagutina I, Perota A, Duchi R, Quadalti C, Lazzari G, Galli C. Xenotransplantation (2015) 22: S180
  • 279 PERV (Porcine Endogenous Retrovirus) integration and expression in pancreatic islets and other tissues in neonatal and adult pigs Nizar Mourad, Claire Crossan, Victoria Cruikshank, Pierre Gianello, Linda Scobie Xenotransplantation (2015) 22 :

IETS 2016

  • Holding pig oocytes at 24°C prior to in vitro maturation alters the developmental capacity after in vitro fertilization, but not parthenogenetic activation. Quadalti C, Lagutina I, Lazzari G, Galli C. Reproduction, Fertility and Development (2015) 28(2): 130 - 260

TT2016

  • Generation of Gal-KO bovine fibroblast colonies using CRISPR/Cas9 technology and magnetic beads selection. Perota A, Lagutina I, Quadalti C, Duchi R, Turini P, Crotti G, Colleoni S, Lazzari G, Galli C. Transgenic Res (2016) 25:256
  • Use of CRISPR/Cas9 technology combined with ssODNs to obtain a specific mono-allelic amino acid substitution in the TDP43 protein for the generation of a swine model of Amyotrophic Lateral Sclerosis (ALS). Quadalti C., Perota A, LagutinaI, Lazzari G, Crociara P, Chieppa MN, Casalone C, Corona C, Bonetto V, Galli C. Transgenic Res (2016) 25:257

Organization of conference

  • Avidin organized a Miniconference & Workshop entitled “TOP LIFE SCIENCE METHODS - Label-free and traditional methods to analyze cellular response to natural and druglike compounds” at Avidin’s headquarters on 6th July 2015. More than 30 participants, including university students, post-docs and senior scientists took theoretical and practical courses on traditional biochemical and FACS methods, 3D cell cultures for screening and diagnostics and holographic 3D microscopy.

 

This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 601827